FastQCFastQC Report
Fri 25 Mar 2016
C8C2NACXX_l06n01_sea159.3510000004e11b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea159.3510000004e11b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287750
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC474522.0741776854988525TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT51400.042.53791414
GCACACG52000.042.35267611
AGTCACG51650.042.1151828
GATCTCG51350.042.10666739
CACACGT52150.042.10144412
GTCACGA51750.042.033829
ACACGTC52150.042.0151713
TCGTATG52200.041.84929743
CTCGTAT51750.041.82285342
CGATCAG51950.041.71609533
CACGATC51950.041.66366231
CGTATGC52500.041.6530144
CGTCTGA52300.041.6338616
GTATGCC52500.041.61015745
CCAGTCA52400.041.51238626
CAGTCAC52450.041.47281327
ACGTCTG52700.041.44590415
GATCGGA53350.041.4105451
ACTCCAG52800.041.36831323
CAGATCT51850.041.36614237
Produced by FastQC (version 0.11.2)