FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l06n01_sea204.3510000004e581.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea204.3510000004e581.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40738
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG10422.557808434385586TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA850.045.04921
TCGGAAG850.045.04923
ATCGGAA850.045.04922
GTCACGT800.044.99385529
CAGTCAC800.044.99385527
CACACGT850.044.99385512
CCAGTCA800.044.99385526
ACGTCCG850.044.99385532
CACGTCC850.044.99385531
CCGCACA800.044.99385536
TCACGTC850.044.99385530
AGCACAC850.044.99385510
TCCGCAC800.044.99385535
CGCACAT800.044.99385537
GCACACG850.044.99385511
AGTCACG800.044.99385528
CGTCTGA850.044.99385516
ACGTCTG950.042.62575515
CTGAACT900.042.49419819
GAACTCC900.042.49419821
Produced by FastQC (version 0.11.2)