FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l06n01_sea200.3510000004e5fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea200.3510000004e5fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39541
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG11842.9943602842619055TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1300.043.2692312
CACGTCT1300.043.2692314
CGGAAGA1300.043.269234
ACACGTC1350.041.66666413
TCGGAAG1350.041.6666643
AGAGCAC1350.041.6666648
ACGTCTG1300.041.5384615
GATCGGA1300.041.538461
GTCTGAA1300.041.5384617
CGTCTGA1300.041.5384616
AACTCCA1200.041.24999622
GAACTCC1200.041.24999621
CCAGTCA1150.041.08695626
GCACACG1400.040.17857411
CTGAACT1350.040.019
ATCGGAA1350.040.02
TGAAACG1250.039.60000235
CAGTCAC1200.039.37499627
CTCCAGT1200.039.37499624
ACTCCAG1200.039.37499623
Produced by FastQC (version 0.11.2)