FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l06n01_sea178.3510000004e284.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea178.3510000004e284.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4665125
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC361300.7744701374561239TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC38200.040.4003431
CGTATGC38900.039.38628844
ACTAGCT39550.039.02968232
GTATGCC39450.038.95123745
CTCGTAT39450.038.60906242
TCGTATG40050.038.25534443
TAGCTTA41550.037.04467434
CTAGCTT41750.036.8133133
ACACGTC42000.036.79587613
TCACTAG42100.036.76190630
ACGTCTG42200.036.51448415
CACGTCT42300.036.4813414
GCACACG42450.036.4058111
CACACGT42700.036.19266512
GCTTATC43100.035.66137336
CGTCTGA43750.035.37507616
ACTCCAG44100.035.04331223
CAGTCAC44100.034.89028527
CCAGTCA44600.034.65045526
CTTATCT44100.034.5975837
Produced by FastQC (version 0.11.2)