FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l06n01_sea159.3510000004e11b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea159.3510000004e11b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16833
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC297817.69143943444425TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGAA3400.045.00000417
CTGAACT3400.045.00000419
TCTGAAC3400.045.00000418
TGAACTC3400.045.00000420
ACACGTC3550.045.013
CACACGT3550.045.012
ACGTCTG3500.045.015
CACGTCT3550.045.014
GATCGGA3550.045.01
TCGGAAG3550.045.03
CGGAAGA3550.045.04
AGAGCAC3600.045.08
ATCGGAA3550.045.02
GGAAGAG3550.045.05
AGCACAC3600.045.010
GCACACG3600.045.011
CGTCTGA3450.044.99999616
GAGCACA3650.044.3835649
GAACTCC3450.044.34782421
AAGAGCA3700.043.7837877
Produced by FastQC (version 0.11.2)