FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l05n01_sea198.3510000004e55b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l05n01_sea198.3510000004e55b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6284733
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG1926823.0658740792966066TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC240250.043.271513
CACACGT240900.043.18276212
GTCCCGA224400.043.15735636
CCGTCCC233850.042.981434
CACGTCT242000.042.930714
GCACACG242650.042.92695211
CGTCCCG232650.042.8839335
CCCGTCC236200.042.71469533
TCGTATG223250.042.6411945
CTCGTAT223700.042.62581644
ACGTCTG243950.042.54142415
AGTCACC239200.042.5118628
CACCCGT238300.042.4967631
AGCACAC245750.042.4868310
ACCCGTC238500.042.40485832
TCTCGTA224350.042.30174343
TCGGAAG247400.042.264393
CGTCTGA245700.042.23842216
GAACTCC243850.042.2175121
GATCGGA247200.042.213271