FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l05n01_sea177.3510000004e65a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l05n01_sea177.3510000004e65a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16478701
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG5808923.5251079560215337TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG690050.043.3732611
ACGTCTG681400.043.31954615
ACACGTC683350.043.29153413
CACACGT692600.043.10313412
CACGTCT685250.043.08272614
TCGTATG678000.042.99848645
AGTCACG685000.042.9803528
GATCGGA703250.042.9608731
CGTCTGA687050.042.94038416
GTCACGT686400.042.6796529
AGCACAC706550.042.4887610
ACTCCAG687500.042.4608423
GAACTCC691300.042.39652621
CTCGTAT676250.042.3944244
TCGGAAG714350.042.37193
GATCTCG677450.042.27130541
CAGTCAC694350.042.25902627
ACGATCT663300.042.23726339
CGATCTC664850.042.13438440
CCAGTCA694100.042.12515326