FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l05n01_sea176.3510000004e5ee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l05n01_sea176.3510000004e5ee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20336128
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG4736142.3289290862055942TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC570600.042.65234813
CACACGT571750.042.60590712
GCACACG573400.042.4872311
ACGTCCG563300.042.3042232
AGTCACG571700.042.28301228
TCGTATG567950.042.2813645
CACGTCC564400.042.10051731
GATCGGA589000.042.0871851
CACGTCT577750.042.03872714
CGTCCGC563550.041.9952333
GTCCGCA562400.041.96652634
CGCACAT565300.041.85565637
AGCACAC583950.041.81270610
ACGTCTG584500.041.7957415
GTCACGT575450.041.77290329
CTCGTAT573200.041.70961844
TCGGAAG597400.041.6714863
CGTCTGA586350.041.64084616
CAGTCAC580100.041.61645527
TCCGCAC565400.041.54088235
Produced by FastQC (version 0.11.2)