FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l05n01_sea171.3510000004e4d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l05n01_sea171.3510000004e4d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7433992
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG3769795.071017025576568TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG437450.043.7644911
CACACGT437000.043.73748412
GATCGGA445650.043.704321
ACACGTC437250.043.70218713
TCGGAAG447950.043.5588043
CACGTCT438150.043.5405414
ACGTCTG442600.043.2298415
TCGTATG428350.043.1766845
AGCACAC444650.043.12724310
CGTCTGA444750.042.9905116
AGAGCAC449450.042.92548
CAGTCAC441350.042.77628327
CGGAAGA456100.042.746494
CTCGTAT432350.042.65232544
ACTCCAG442600.042.6453123
ATCGGAA458000.042.604462
GAACTCC444400.042.5890121
CCAGTCA441650.042.58422526
CTCCAGT442550.042.5840424
GAGCACA452700.042.5575949
Produced by FastQC (version 0.11.2)