FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l05n01_sea169.3510000004e3d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l05n01_sea169.3510000004e3d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14660617
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC30350.019.42051313
CACACGT31150.018.7050912
GCACACG31700.018.59345611
CGTATGC30650.017.39771744
TCGTATG30500.017.335843
CTCGTAT29800.017.29006242
GTATGCC31650.016.91905645
AGTCACC32900.016.75294928
CACGTCT35150.016.57648814
GTCACCT33800.016.37342329
ACGTCTG38450.015.21230715
CAGTCAC37550.014.91799627
CCTTGTA34950.014.80775233
ACCTTGT37400.014.25853332
AGCACAC43100.014.2498710
CGTCTGA42100.014.16060716
GATCGGA41800.013.8933651
GAACTCC43900.013.63123621
CCAGTCA41600.013.57380126
CACCTTG40200.013.32073831