FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l05n01_sea168.3510000004e369.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l05n01_sea168.3510000004e369.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15850381
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC10405966.565116636628482TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1182550.043.9275111
CACACGT1180500.043.9085112
ACACGTC1181100.043.8785813
GTATGCC1144700.043.7906545
CACGTCT1185050.043.69597614
ACGTCTG1185450.043.6337915
ACGGCTA1164650.043.5226732
CGTCTGA1187900.043.4850916
AGTCACG1173450.043.4722528
AGCACAC1196250.043.4550710
GGCTACA1155800.043.40702434
CGGCTAC1160750.043.39777433
GAACTCC1181400.043.29968321
GTCACGG1177000.043.285729
CACGGCT1171250.043.27948831
GCTACAT1159450.043.24902335
CGTATGC1158350.043.24548344
TCACGGC1173500.043.22514730
CTCGTAT1155300.043.20009242
TCGTATG1161050.043.19530543