FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l05n01_sea167.3510000004e2e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l05n01_sea167.3510000004e2e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16861980
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC28000.023.54384431
CGTATGC32500.020.76871344
ACTAGCT31950.020.56359732
GTATGCC35100.019.4225945
ACGTCTG37050.019.00514215
GCACACG38400.018.86425411
CTCGTAT38200.018.02311142
ACACGTC40250.017.94130913
CACGTCT40150.017.87393214
TCGTATG38650.017.81326743
CACACGT40900.017.6561812
TCACTAG38900.017.11836430
TAGCTTA38750.017.07236534
CTAGCTT39800.016.6214233
CGTCTGA44100.016.32399416
GCTTATC42450.015.90283336
CAGTCAC48350.014.61001727
GAACTCC51250.014.35386721
ACTCCAG51500.014.28427123
CCAGTCA49600.014.06039726
Produced by FastQC (version 0.11.2)