FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l05n01_sea166.3510000004e26a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l05n01_sea166.3510000004e26a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16370602
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC20900.012.91886144
GCACACG26350.011.52554611
GTATGCC23500.011.29804945
AGTCACG24100.010.82812728
ACACGTC29200.010.785830513
ACGTCTG28950.010.72355815
TCGTATG28100.010.08912643
CACGTCT31100.09.83754814
CTCGTAT27850.09.61421242
CGTCTGA33850.09.37063116
CACACGT33900.09.09136912
GTCACGA28200.08.69540429
CACGATC30250.08.4792931
CCAGTCA35750.08.30635126
GATCTCG32000.08.22782239
ACTCCAG38700.08.0219623
CAGTCAC34050.07.994303727
GAACTCC41450.07.381128321
TCCAGTC43650.07.266867625
AGCACAC44600.07.26342310
Produced by FastQC (version 0.11.2)