FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l05n01_sea165.3510000004e1e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l05n01_sea165.3510000004e1e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20205412
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC5264072.6052772395831374TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC593500.042.9962313
GCACACG597550.042.8441211
ACGTCTG595350.042.7869415
CACACGT597850.042.77746212
GTATGCC584950.042.71379545
CACGTCT598400.042.61397614
CGTATGC587200.042.56162344
CGTCTGA600600.042.38296516
CTCGTAT589950.042.29468542
GTCACAC594600.042.14106429
TCGTATG594050.042.13154643
GATCGGA610800.042.0049131
GAACTCC607750.041.77329621
AGCACAC613800.041.75811810
CAGTCAC603250.041.7083527
TCGGAAG618250.041.611173
CCAGTCA605050.041.56196226
ACTCCAG609550.041.51717423
TCCAGTC609900.041.34211325
TCTCGTA606100.041.2618441
Produced by FastQC (version 0.11.2)