FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l05n01_sea164.3510000004e16b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l05n01_sea164.3510000004e16b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9276705
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC13550.015.44263244
GCACACG16650.015.40340511
GTATGCC14750.014.49136345
ACACGTC17650.014.02084213
TCGTATG15400.013.87971543
ACGTCTG18050.013.83469515
CACGTCT18700.013.35380914
CTCGTAT16600.013.28298442
CACACGT19400.012.87204112
CGTCTGA21700.011.71500516
CAGTCAC19600.011.36325827
ACTCCAG24350.011.08679723
CTGACCA19300.010.84266533
ACTGACC19950.010.60189932
CCAGTCA22950.010.48877826
TCCAGTC24250.010.39034725
AGCACAC26300.010.17945110
GAACTCC26000.010.03710421
GTCACTG24350.09.23899729
CACTGAC25200.09.01765831