FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l05n01_sea163.3510000004e10e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l05n01_sea163.3510000004e10e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7805545
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC4016925.1462389877964965TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTAG430900.044.22790531
ACTTAGG431250.044.15661632
ACGTCTG437850.043.9399415
GTATGCC432400.043.9273745
GCACACG439200.043.92268811
ACACGTC438000.043.90434613
CACGTCT438500.043.85428214
CACACGT440100.043.80219712
CGTATGC433550.043.78490444
CTCGTAT432150.043.7604242
AGGCATC430100.043.76040336
CAGTCAC438700.043.71177727
CGTCTGA439750.043.70404416
GGCATCT430600.043.6834637
TCGTATG435650.043.6358243
CCAGTCA440100.043.55739226
GATCGGA441300.043.5558241
TTAGGCA435600.043.4604534
ACTCCAG443550.043.38089823
CATCTCG436150.043.33940539