FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l05n01_sea162.3510000004e0a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l05n01_sea162.3510000004e0a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15404718
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC3958872.5699074789944225TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG450650.042.9912711
ACGTCTG449500.042.9210815
CGTATGC441850.042.88652444
GTATGCC441700.042.88580745
ACACGTC450850.042.872413
CACGTCT451150.042.75413514
GTCACCG445050.042.7488629
CACACGT454350.042.64116712
CGTCTGA454150.042.46676316
TCGTATG446850.042.42678543
AGTCACC449150.042.38868728
CTCGTAT446850.042.29586842
GATCGGA460950.042.108631
CGATGTA449850.042.10211634
CCGATGT450450.042.10002533
CAGTCAC456250.041.83752427
GAACTCC458450.041.72985521
AGCACAC464700.041.70234710
ACTCCAG458400.041.67074623
GTATCTC455700.041.6323838