FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l05n01_sea161.3510000004e043.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l05n01_sea161.3510000004e043.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13966004
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC5528323.9584121556889147TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG615750.043.40374811
ACGTCTG613550.043.2805615
CACGTCT615250.043.259714
ACACGTC616450.043.25592813
GTATGCC603000.043.1415945
CGTCTGA616400.043.0731516
CACACGT620650.043.00670612
CGTATGC607350.042.84371644
CACGATC603950.042.75216736
GATCGGA629550.042.7053261
GATCTCG608900.042.61557839
ACGATCT608000.042.57842337
CTCGTAT610200.042.55910542
TCGTATG612350.042.51593443
AGCACAC630000.042.47305310
CGATCTC609350.042.4730138
CCAGTCA616300.042.45974726
TCGGAAG635000.042.4418563
GAACTCC623150.042.40802821
CAGTCAC618800.042.33910827