FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l04n01_ah8643i-0303166.3510000004e4c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l04n01_ah8643i-0303166.3510000004e4c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14039005
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG8523336.071178121241498TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA953850.044.472111
GCACACG947500.044.15284711
CACACGT953450.043.87731612
TCGGAAG964650.043.873
AGCACAC956750.043.84290310
ACACGTC954350.043.81456413
GAGCACA958250.043.753929
CACGTCT954950.043.70914514
ACGTCTG952550.043.69174615
AGAGCAC962200.043.6608168
CGTCTGA954650.043.5461516
ATCGGAA974850.043.5302662
GAACTCC957600.043.13480421
CGGAAGA980200.043.1155664
TCGTATG945100.043.0836145
TCCAGTC958950.043.03185325
CAGTCAC960450.043.00787727
AGTTCCG949550.042.99431234
GTTCCGT948700.042.9477435
GTCACAG959500.042.9426129
Produced by FastQC (version 0.11.2)