FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l04n01_ah8643a-030316.3510000004e432.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l04n01_ah8643a-030316.3510000004e432.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17906740
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG2681591.4975310972293114TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG316800.042.25036211
GATCGGA323150.041.8876461
CACACGT321950.041.54656212
ACACGTC321700.041.4525213
CACGTCT323200.041.15492614
AGCACAC327950.040.9053510
ACGTCTG324450.040.89237215
GAGCACA329950.040.7053539
CGTCTGA326600.040.65761616
GAACTCC328200.040.10299721
TCGGAAG338350.039.96623
TCCAGTC331650.039.6044325
CACAGTC329050.039.5430431
AGAGCAC340650.039.5390478
CAGTCAC332550.039.5185327
CCAGTCA332300.039.50742726
TCGTATG333350.039.1677345
AGTCACA335500.039.15764628
ATCGGAA346600.039.09912
GTCTGAA340350.039.00845317
Produced by FastQC (version 0.11.2)