FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l04n01_ah8630i-030316.3510000004e3b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l04n01_ah8630i-030316.3510000004e3b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13142561
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC4619923.5152357291702887TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG513150.044.0843611
GATCGGA519550.043.8014531
CACACGT516300.043.76310712
ACACGTC516500.043.7066313
AGCACAC519300.043.60291710
ACGTCTG516300.043.600915
CACGTCT519550.043.4450614
CGTCTGA519700.043.25072516
GAGCACA524900.043.1641049
TCGGAAG528050.043.1311383
AGAGCAC526750.043.0552148
CAGTCAC521200.042.885227
GAACTCC521150.042.87139521
GTATGCC518750.042.82686245
TCCAGTC523100.042.7760925
CCAGTCA524500.042.60251226
AGTCACC523300.042.60133428
CGTATGC523650.042.43997244
GTCACCT525400.042.38395729
ACTCCAG527800.042.3823823
Produced by FastQC (version 0.11.2)