FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l04n01_ah8630a-030316.3510000004e333.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l04n01_ah8630a-030316.3510000004e333.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17978615
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG23650.014.74330311
CACACGT28250.012.50188412
ACACGTC28200.012.44410813
GATCGGA31000.011.5444321
ACGTCTG30150.011.48994615
CACGTCT31000.011.465157514
AGCACAC32300.010.72594210
CGTCTGA34350.010.34701216
CATCTCG28900.010.12411939
GAGCACA36200.09.8812489
GTATGCC33250.09.81183745
GAACTCC37350.09.63638121
TCCAGTC40000.09.05420925
CAGTCAC37550.08.80656427
TCGGAAG46450.08.5284513
CGTATGC38000.08.52633944
CCAGTCA41900.08.48283426
AGAGCAC43600.08.1525578
AGTCACC41600.08.00327128
CTCGTAT38300.07.931006442