FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l04n01_ah8584b-030316.3510000004e6aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l04n01_ah8584b-030316.3510000004e6aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16545123
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1074610.6495025754719381TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG134100.040.59625611
ACACGTC137800.039.53851713
GATCGGA140550.039.282081
ACGTCTG139550.038.8647815
CACACGT141500.038.58448812
CACGTCT141600.038.42921414
AGCACAC144200.037.83209210
CGTCTGA144800.037.50227416
CAGTCAC145850.036.58516727
GAGCACA151150.036.4056439
GAACTCC147550.036.28495821
TCGGAAG153500.035.997933
TCGTATG147650.035.68855745
CTCGTAT147550.035.63714644
TCCAGTC150300.035.51629325
AGAGCAC155350.035.421398
CCAGTCA150950.035.3043926
TCACATG152150.034.99838630
GTCTGAA155850.034.98764417
GTCACAT152750.034.7558229
Produced by FastQC (version 0.11.2)