FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l04n01_ah8584a-030316.3510000004e623.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l04n01_ah8584a-030316.3510000004e623.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12807006
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG405290.31645960031563974TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG49550.040.74179535
GTCCCGA51800.038.66995636
CCCGTCC55250.037.35223833
ACCCGTC55750.037.0581832
GCACACG59150.036.77673711
CCCGATC54550.036.71993638
CCGTCCC57250.035.8510834
TCACCCG57950.035.67719330
GATCGGA63150.034.6436271
ACGTCTG63000.034.45731715
CACCCGT60150.034.38128731
ACACGTC64450.033.7172613
TCCCGAT59500.033.70343837
CACACGT64850.033.5789312
CACGTCT65250.033.37256214
CCGATCT60000.033.2720139
CGATCTC60400.033.20032540
CGTCTGA65600.032.9201716
GATCTCG61250.032.4420841
GTCACCC65500.031.80334929
Produced by FastQC (version 0.11.2)