FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l04n01_ah8428i-030316.3510000004e2ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l04n01_ah8428i-030316.3510000004e2ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13370752
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC2314101.73071791324826TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG269250.043.21985611
GATCGGA274650.042.9415051
GTCACGC270150.042.70979729
CACACGT272350.042.6618312
ACACGTC273400.042.45652813
ACGTCTG274150.042.35631615
TCACGCC272500.042.2049230
AGTCACG274200.042.18561628
CACGTCT275600.042.10898214
CGTCTGA277050.041.89671716
AGCACAC278350.041.85678510
CACGCCA274850.041.71520631
GAGCACA283100.041.2743039
CAGTCAC281000.041.2688327
TATCTCG280700.041.03666739
GTATGCC280250.041.0258845
TCGGAAG288000.040.9750483
TCCAGTC283350.040.8857925
CCAGTCA284200.040.7565126
CGTATGC282350.040.7061844
Produced by FastQC (version 0.11.2)