FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l04n01_ah8428a-030316.3510000004e234.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l04n01_ah8428a-030316.3510000004e234.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15837101
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC6430224.060225416255159TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA718950.043.935161
GCACACG710100.043.8898711
CACACGT716750.043.50149512
ACACGTC717600.043.4616813
ACGTCTG714200.043.4030415
CACGTCT719300.043.28920414
AGCACAC722950.043.1577910
GAGCACA724750.043.1407629
CGTCTGA718950.043.08499516
AGAGCAC727500.043.024078
TCGGAAG734300.042.9678233
GTCACAC717450.042.81898529
GATCTCG715550.042.79193539
GTATGCC717450.042.7477545
ATCGGAA739450.042.729312
GAACTCC720350.042.6827321
CACACAG719200.042.6546731
CAGTCAC723400.042.49167327
TCCAGTC724100.042.44304325
GTCTGAA730350.042.33856217