FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l04n01_ah7661d-030316.3510000004e5ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l04n01_ah7661d-030316.3510000004e5ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15783243
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG662620.41982500047677146TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG88650.038.44445811
GATCGGA91750.037.386791
AGTCACG89200.037.3752928
ACACGTC92750.036.7205413
ACGTCTG92500.036.69795615
CACGTCT93700.036.2759814
GTCACGT92600.035.83292429
CACACGT95400.035.7243312
CGTCTGA95200.035.51537316
AGCACAC98400.034.86489510
TCACGTG96300.034.598330
CGATCTC93700.034.5392740
CACGTGA96600.034.3141131
GATCTCG95250.034.2332241
GAGCACA102550.033.4323589
GAACTCC101400.032.90012421
TCCAGTC102200.032.73063325
AGAGCAC105900.032.417268
CAGTCAC103700.032.3661727
TCGGAAG108050.032.0178763
Produced by FastQC (version 0.11.2)