FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l04n01_ah7661a-030316.3510000004e54e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l04n01_ah7661a-030316.3510000004e54e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15358571
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG323550.21066413014596214TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG45500.034.72217632
CGTCCGC47150.033.45902333
GCACACG50200.033.11655411
AGTCACG51900.030.99143828
GTCCGCA53000.030.02077934
CACGTCC53300.030.01854531
CCGCACA52600.029.99431436
ACGTCTG56150.029.60664715
GATCGGA58650.029.1652011
CACACGT57350.029.0270412
ACACGTC57050.028.98242413
GTCACGT55450.028.52047729
AGCACAC59400.028.17785310
TCACGTC57100.028.01360730
CGTCTGA59450.027.92537916
TCCGCAC56500.027.84288235
CACGTCT59900.027.67803214
GAGCACA62500.026.9604689
CATCTCG60050.026.53245441
CAGTCAC61400.026.19634227
Produced by FastQC (version 0.11.2)