FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l04n01_ah7660i-030316.3510000004e1bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l04n01_ah7660i-030316.3510000004e1bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14285645
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC4090902.86364388867286TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG450650.043.69288311
GATCGGA456350.043.583941
CACACGT454550.043.3229512
ACACGTC454900.043.26968813
ACGTCTG453650.043.26944715
AGCACAC456750.043.14071710
CACGTCT456450.043.06808514
CGTCTGA456700.042.92629616
GAGCACA462400.042.691879
TCGGAAG468050.042.4949043
ACTGACC457100.042.4327632
AGAGCAC466400.042.373978
GTATGCC454350.042.35108645
GAACTCC461850.042.2430721
CAGTCAC461100.042.23017527
CCAGTCA463050.042.06661226
TCCAGTC464650.041.97882525
CGTATGC458700.041.96549644
AGTCACT464700.041.87881528
GTCTGAA467700.041.86860317
Produced by FastQC (version 0.11.2)