FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l04n01_ah7660a-030316.3510000004e135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l04n01_ah7660a-030316.3510000004e135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17570843
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC4559552.594952330972396TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG499850.043.35370611
GATCGGA509050.043.04361
CACACGT503650.042.96407312
ACACGTC503250.042.94880713
ACGTCTG504400.042.7522815
CACGTCT506950.042.62154414
AGCACAC510650.042.51803210
CGTCTGA509450.042.25342616
GAGCACA515450.042.2315649
TATCTCG506300.042.09104539
CCGATGT506550.042.01119633
AGAGCAC519500.041.95438
GAACTCC509750.041.89758321
GTATGCC507900.041.81768445
TCGGAAG524750.041.7475553
CGATGTA510600.041.64341734
TCCAGTC512750.041.62173525
CAGTCAC513250.041.56898527
CGTATGC512050.041.4976444
GTCACCG514000.041.4164229
Produced by FastQC (version 0.11.2)