FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l03n01_ah8585b-030316.3510000004e192.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l03n01_ah8585b-030316.3510000004e192.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16565140
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG10501786.3396868363322TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1224150.044.4108541
GCACACG1213500.044.0811511
ACACGTC1213450.043.85466413
CACACGT1215550.043.84579512
AGCACAC1223900.043.84129710
TCGGAAG1238400.043.8030133
GAGCACA1231650.043.7493029
ATCGGAA1243100.043.7176782
CACGTCT1215950.043.67569414
ACGTCTG1218000.043.6354315
AGAGCAC1237000.043.6166088
CGTCTGA1220750.043.5113316
CGGAAGA1248750.043.3846244
GAACTCC1223400.043.2773321
GTCTGAA1235700.043.0686617
TCGTATG1196400.043.0018945
TCCAGTC1227050.042.9817625
ACTCCAG1231150.042.87882223
TCTGAAC1240750.042.8335318
CAGTCAC1234800.042.78122327
Produced by FastQC (version 0.11.2)