FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l03n01_ah8585a-030316.3510000004e128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l03n01_ah8585a-030316.3510000004e128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17727417
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG3052511.7219147042121252TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG361850.042.49407611
GATCGGA367100.042.4062731
ACACGTC364250.042.07785413
ACGTCTG363450.042.00335315
CACACGT366550.041.92465612
CGTCTGA366050.041.6558416
CACGTCT367350.041.6064114
AGCACAC372450.041.3770410
GAGCACA375250.041.1500639
GAACTCC372250.041.0043521
AGTTCCG360900.040.93974334
TATCTCG361500.040.8747941
CCGTATC362700.040.80228438
AGAGCAC379200.040.7630738
CAGTCAC374000.040.59593627
TCGGAAG384500.040.5379683
TCCAGTC375950.040.49906525
GTTCCGT365900.040.22680735
CCAGTCA378550.040.15553726
CGTATCT370100.040.11367839
Produced by FastQC (version 0.11.2)