FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l03n01_ah8583b-030316.3510000004e0d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l03n01_ah8583b-030316.3510000004e0d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13435553
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC266550.19839153624714967TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG40800.033.35903511
ACACGTC41850.031.984413
ACGTCTG42600.031.36848415
CACACGT43100.031.10908912
CACGTCT44900.029.76163314
GATCGGA47450.028.9902211
CATCTCG45200.028.62366939
AGCACAC48500.028.24934610
CGTCTGA49150.027.37123116
CGTATGC47900.026.86820244
GAACTCC50450.026.53215221
CAGTCAC51250.026.2496827
TCCAGTC51700.026.2387725
GAGCACA52850.026.0962099
TCGGAAG54450.025.7199293
GTATGCC50850.025.3973145
CTCGTAT51850.025.12538542
CCAGTCA54850.024.69087826
TCGTATG53200.024.48753443
AGTCACC56350.024.03362328
Produced by FastQC (version 0.11.2)