FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l03n01_ah8583a-030316.3510000004e093.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l03n01_ah8583a-030316.3510000004e093.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15349483
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2425831.5803985059301344TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG282550.042.70911
GATCGGA287300.042.3635031
CACACGT283400.042.32687812
ACACGTC284150.042.1751613
ACGTCTG286600.041.72029515
AGCACAC289400.041.6995810
CACGTCT288600.041.4545614
CGTCTGA288900.041.3258516
GAGCACA293300.041.2561239
TCCAGTC291400.040.97902725
AGAGCAC296100.040.9191868
GTATGCC283050.040.8325345
GAACTCC294750.040.658321
CAGTCAC293850.040.56080227
CCAGTCA294600.040.49572426
TCGGAAG301300.040.413573
CGTATGC287900.040.37181544
CTCGTAT290700.040.1301442
AGTCACC296850.039.9765828
ATCTCGT293550.039.82535640
Produced by FastQC (version 0.11.2)