FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l03n01_ah8115b-030316.3510000004e06c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l03n01_ah8115b-030316.3510000004e06c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14883593
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC3897112.618393287158551TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG444750.043.9317211
ACACGTC447950.043.45171713
CACACGT449600.043.3927612
CACGTCT449950.043.2184314
ACGTCTG451050.043.1579215
AGCACAC454200.043.12227610
GATCGGA460600.042.9971851
GAGCACA458000.042.8524679
TCGGAAG463300.042.736633
TATCTCG448400.042.72851639
GAACTCC453600.042.72683721
CCGATGT444650.042.67988633
GTATGCC441400.042.6336745
CAGTCAC454400.042.4288227
TCCAGTC457700.042.2507125
AGTCACC456800.042.23545528
CCAGTCA458200.042.1702426
CGATGTA450950.042.14848334
GTCTGAA461750.042.09937317
ACCGATG451450.042.0717632