FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l03n01_ah8115a-030316.3510000004dff4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l03n01_ah8115a-030316.3510000004dff4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15259126
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC366460.24015792254418766TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG52950.035.17951611
CACACGT59000.031.68651612
ACACGTC59550.031.318113
ACGTCTG60850.030.7599315
GATCGGA60900.030.7201841
CGTCTGA61500.030.39824716
CACGTCT61650.030.36077514
AGCACAC64200.029.22580110
ACTGACC63000.028.67724832
CAGTCAC65150.028.41895127
GAACTCC66400.028.29052721
TCCAGTC67150.027.7065325
GTATGCC64600.027.65343345
GAGCACA68450.027.5451959
CTCGTAT66600.026.99252942
CGTATGC66950.026.68338644
CCAGTCA70300.026.46505526
ATCTCGT69100.026.04902540
AGTCACT72050.025.97837428
AGAGCAC73900.025.605128
Produced by FastQC (version 0.11.2)