FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l03n01_ah8104-3-030316.3510000004e390.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l03n01_ah8104-3-030316.3510000004e390.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15497776
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG560980.36197451815021714TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG73000.040.6186311
CACACGT76250.038.59229312
TCACGTG74400.038.52405530
GATCGGA78200.038.2013441
ACACGTC77250.037.94661313
CACGTGA77450.036.68862531
ACGTCTG79700.036.6388715
AGTCACG80600.036.2297528
AGAGCAC82550.036.114188
GAGCACA83350.036.01059
GTCACGT80250.035.9952929
ACTCCAG81000.035.96751423
ACGTGAA79350.035.78235632
AGCACAC85100.035.24092510
CAGTCAC82950.035.12198627
CACGTCT83300.035.10944714
GAACTCC83900.034.80474521
GATCTCG83700.033.30660641
AACGATC84500.033.2310338
TCCAGTC90250.032.35587325
Produced by FastQC (version 0.11.2)