FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l03n01_ah8104-2-030316.3510000004e319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l03n01_ah8104-2-030316.3510000004e319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29931932
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG323820.10818546560910267TruSeq Adapter, Index 18 (97% over 40bp)
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC304780.10182436603156789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG52400.032.7151911
ACGTCCG50400.031.337632
TCCGCAC51400.029.63396535
CACACGT57850.029.6331212
GATCGGA60200.028.7944531
CGCACAT54450.028.30671937
ACACGTC60300.028.20517513
CCGCACA56650.028.16063736
GAACTCC61850.027.0254821
AGTCACG61050.026.90057428
GTCCGCA59450.025.92392534
ACTCCAG64450.025.86542523
CGTCCGC60850.025.54928833
ACGTCTG67050.025.3992815
CCCGGGT12800.025.1501541
GTCACGT67250.024.35361329
GGGGGGT53200.024.204661
TCACGTC66700.024.1499730
GAGCACA75100.023.8176779
CATCTCG67400.023.30156741
Produced by FastQC (version 0.11.2)