FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l03n01_ah119-3-030316.3510000004e291.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l03n01_ah119-3-030316.3510000004e291.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20681750
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG677460.3275641568049125TruSeq Adapter, Index 16 (97% over 40bp)
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC269820.13046284767971764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG83800.041.58819635
GCACACG93250.040.7723611
GTCCCGA87000.039.31149736
CACACGT97500.038.87973412
GATCGGA99750.038.5463641
TCACCCG95150.037.9490230
ACACGTC102000.037.09810613
CACCCGT97400.036.63519331
ACTCCAG103000.036.38837423
TCCCGAT95000.036.07219337
GAACTCC105250.035.73872421
GAGCACA109000.035.5239459
ACGTCTG106350.035.45368615
AGCACAC109900.035.04657710
ACCCGTC103350.034.43939632
CACGTCT109800.034.31921414
CCCGATC102950.033.6581838
CCCGTCC108350.032.8709733
CAGTCAC113800.032.63846227
AGAGCAC118050.032.514718
Produced by FastQC (version 0.11.2)