FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l03n01_ah119-2-030316.3510000004e21a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l03n01_ah119-2-030316.3510000004e21a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23434192
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG2577921.1000677983691522TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG314550.043.13392311
GATCGGA322150.042.6838261
CACACGT319150.042.31485412
ACACGTC320450.041.9817213
GAACTCC323800.041.533421
GAGCACA331700.041.4244239
ACTCCAG323600.041.336623
TCACATG322150.040.98573330
ACGTCTG328350.040.91675615
AGCACAC335500.040.7577110
AGAGCAC340950.040.2411848
CACGTCT334300.040.13466614
AGTCACA336650.039.8745528
CAGTCAC338700.039.46051827
GTCACAT337150.039.4284129
CACATGT331800.039.36179431
ATGTCAG331450.039.13325534
TCGTATG330850.038.7901545
AACTCCA346850.038.71491222
GTCTGAA348400.038.69764717
Produced by FastQC (version 0.11.2)