FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l02n01_ah8585d-030316.3510000004e574.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l02n01_ah8585d-030316.3510000004e574.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14857636
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG479610.3228037084769071TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG59400.038.4827632
CGTCCGC60900.037.35081533
GCACACG66700.036.901311
ACACGTC70000.035.09738513
AGTCACG66250.034.97911528
CCGCACA66250.034.53914336
CACGTCC66450.034.50151431
CACACGT71400.034.44071212
ACGTCTG71700.034.1709815
CACGTCT72400.033.87167414
GATCGGA74350.033.6937981
TCACGTC68900.033.33987430
AGCACAC74150.033.19475610
GTCCGCA68600.033.06009734
CGTCTGA75050.032.8255616
CATCTCG71350.032.41699241
GTCACGT70950.032.37656829
TCCGCAC71100.031.9921535
GAACTCC76200.031.8282321
GAGCACA80200.030.7475979
Produced by FastQC (version 0.11.2)