FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l02n01_ah8585c-030316.3510000004e4fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l02n01_ah8585c-030316.3510000004e4fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16099832
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG192720.11970311242999307TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG39400.029.52118711
AGTCACG39050.027.25107628
ACACGTC44100.026.47696913
ACGTCTG44650.025.99966615
GATCGGA45550.025.7941551
CACACGT45800.025.44507612
CGATCTC39050.024.89179240
CACGTCT47000.024.7475514
CGTCTGA47300.024.54302416
AGCACAC50000.023.6235410
GTCACGT42850.023.57431629
GATCTCG42900.023.0773641
GAACTCC50850.022.47564921
TCACGTG44650.022.47306830
CACGTGA45300.022.30008731
CAGTCAC50450.022.20797727
GAGCACA52700.022.2007339
CCAGTCA56350.020.3219226
TCGGAAG60250.020.2114873
TCCAGTC56550.020.21026225
Produced by FastQC (version 0.11.2)