FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l02n01_ah8511i-030316.3510000004e475.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l02n01_ah8511i-030316.3510000004e475.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10813454
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG974990.9016453022318307TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG119100.042.54240435
GTCCCGA115400.042.4262436
GCACACG129200.042.1583511
ACACGTC131150.041.54867613
ACCCGTC125200.041.4213232
CACACGT131850.041.39634712
CCCGTCC124550.041.2229733
GATCGGA134250.041.1925741
TCACCCG127500.041.15017730
ACGTCTG131900.041.0899215
CACGTCT132950.041.0361614
CACCCGT128550.040.53440531
CCCGATC119350.040.47576538
AGCACAC135250.040.40746710
CGTCTGA135300.040.1737516
TCCCGAT120900.040.04989637
CCGATCT121150.039.7258239
GAGCACA137950.039.6999869
CCGTCCC128200.039.6984934
CGATCTC122750.039.5196240
Produced by FastQC (version 0.11.2)