FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l02n01_ah8511a-030316.3510000004e3e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l02n01_ah8511a-030316.3510000004e3e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14337883
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG16600.013.55309111
CACACGT20150.011.61193812
ACACGTC20000.011.24906513
GATCGGA22300.010.2953991
ACGTCTG22400.010.14417615
CACGTCT23550.09.5532814
AGCACAC26050.09.41390410
CGTCTGA24200.09.29668316
GAACTCC26550.08.6432921
GAGCACA28950.08.3159519
TCGGAAG34200.07.3716913
TCCAGTC29200.07.16545125
GTCTGAA34850.06.907555617
AGAGCAC35650.06.56375038
CCAGTCA33650.06.48532326
GTATGCC28750.06.26085245
CAGTCAC26750.06.223760627
CATCTCG21150.06.170238539
CGTATGC30950.06.106606544
TCGTATG35350.05.919360643