FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l02n01_ah7797i-030316.3510000004e35d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l02n01_ah7797i-030316.3510000004e35d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16482969
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1985381.2045038730583064TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG239500.042.4414511
GATCGGA244100.042.0094761
ACACGTC244250.041.64371513
ACGTCTG244350.041.57092315
CACACGT245350.041.49368312
CACGTCT246450.041.1893114
AGCACAC248250.041.054910
CGTCTGA248150.040.87993616
GAGCACA253050.040.3759089
GAACTCC249750.040.0054821
CAGTCAC249250.039.8782527
TCGGAAG258050.039.775293
TCCAGTC250500.039.58932525
AGAGCAC258850.039.4712148
CCAGTCA253650.039.07118626
TCACATG248700.039.02633730
AGTCACA255450.038.90226728
CACATGT248950.038.86144631
GTCTGAA262500.038.72229817
ACTCCAG257350.038.61423523
Produced by FastQC (version 0.11.2)