FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l02n01_ah7797a-030316.3510000004e2d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l02n01_ah7797a-030316.3510000004e2d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17963643
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG3510001.9539466465682935TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG405200.043.15384711
GATCGGA413800.042.7688941
ACACGTC411050.042.6053713
CACACGT414000.042.2963412
ACGTCTG414700.042.10453415
CACGTCT415400.042.071514
AGCACAC418000.041.96296710
GAGCACA419300.041.823769
CGTCTGA418300.041.73679716
AGTTCCG403450.041.45149234
GAACTCC420950.041.1854421
TATCTCG409500.041.18065341
CCGTATC405300.041.1415438
TCGGAAG430800.041.0410843
CAGTCAC420650.041.03832227
AGAGCAC428600.041.000368
GTTCCGT408050.040.9452735
TCCAGTC423000.040.7996925
AGTCACA422050.040.72684528
CCAGTCA425650.040.5562526
Produced by FastQC (version 0.11.2)