FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l02n01_ah7011i-030316.3510000004e25e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l02n01_ah7011i-030316.3510000004e25e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16948486
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC424645625.055075715907606TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4693700.044.882321
TCGGAAG4700550.044.7265663
ATCGGAA4713550.044.6818472
GCACACG4667000.044.6416511
GAGCACA4687700.044.625219
AGAGCAC4696400.044.593818
AGCACAC4679300.044.5864210
CACACGT4673000.044.56362512
CGGAAGA4714050.044.5608064
ACACGTC4669800.044.53153213
CACGTCT4670500.044.46363414
ACGTCTG4677300.044.4350715
GAAGAGC4733500.044.3751346
CGTCTGA4685250.044.3539116
GGAAGAG4746550.044.3258745
GTATGCC4614550.044.26110545
GTCTGAA4704150.044.2546217
AAGAGCA4748700.044.209757
GAACTCC4658550.044.18023321
TGAACTC4687950.044.10040320
Produced by FastQC (version 0.11.2)