FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l02n01_ah7011a-030316.3510000004e1d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l02n01_ah7011a-030316.3510000004e1d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16559890
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2171871.3115244122998402TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG257050.042.3703911
GATCGGA263450.041.8888931
CACACGT260950.041.7802612
ACACGTC262150.041.52893413
ACGTCTG263600.041.24062315
GTCACAC255350.041.19040329
CACGTCT264100.041.188114
AGCACAC266450.040.93571510
GATCTCG258350.040.63727639
CGTCTGA267650.040.56614716
GAGCACA269900.040.5140729
GTATGCC263600.040.28885345
GAACTCC268500.040.0941821
CACACAG262550.039.91647331
TCGGAAG277300.039.7578123
CAGTCAC270500.039.56485727
TCCAGTC271150.039.4700125
CTCGTAT269250.039.3092442
AGAGCAC278550.039.3045468
AGTCACA271150.039.10552228
Produced by FastQC (version 0.11.2)