FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l02n01_ah6408i-030316.3510000004e15f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l02n01_ah6408i-030316.3510000004e15f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27453819
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG283004410.308380047234959TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3208200.044.5262681
TCGGAAG3221400.044.2354133
GCACACG3187550.044.2241811
AGCACAC3202550.044.1137110
ATCGGAA3237800.044.100442
CACACGT3198900.044.08274512
ACACGTC3197950.044.0402613
GAGCACA3217450.044.0393759
CACGTCT3198150.043.94165414
ACGTCTG3198100.043.9219415
AGAGCAC3230250.043.9171038
CGGAAGA3246100.043.849914
CGTCTGA3209550.043.7372116
GTCTGAA3225600.043.56701317
GAACTCC3194000.043.52540221
TCGTATG3158750.043.45406345
ACTCCAG3198300.043.30790723
TCCAGTC3195950.043.30173525
TGAACTC3228950.043.20826720
GAAGAGC3292800.043.2001726
Produced by FastQC (version 0.11.2)