FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l02n01_ah6408a-030316.3510000004e0bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l02n01_ah6408a-030316.3510000004e0bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25111378
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC3968501.5803593096324704TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG450950.042.5967311
ACACGTC457150.041.81231713
CACACGT458200.041.80488212
ACGTCTG459150.041.58069215
GATCGGA468350.041.2958221
CACGTCT463100.041.25032414
AGCACAC467800.041.1450710
CGTCTGA464850.041.0853516
GAGCACA471500.040.867019
GTATGCC460800.040.48865545
CAGTCAC468150.040.3009127
AGAGCAC479800.040.188278
GAACTCC473050.040.1448821
TCCAGTC472250.039.98429525
CGTATGC468150.039.8625944
CCAGTCA474000.039.76555326
AGTCACC469950.039.56775728
CTCGTAT471850.039.52553642
TCGGAAG489650.039.524313
GTCACCT469250.039.45417829
Produced by FastQC (version 0.11.2)